赌博app

性别: 男
工作电话:020-84113712
职称:副教授
学历: 博士研究生
个人主页://www.researchgate.net/profle/Lichuang-Cao-2/research
电子邮件: [email protected]
导师类型: 硕士生导师
学科方向:071005-微生物学
0860-生物与医药

研究方向

酶工程与合成生物学：（1）极端环境酶资源挖掘；（2）酶工程、酶的结构与功能关系；（3）高性能底盘细胞构建。

个人经历

2022/10-至今，中山大学，赌博app ，副教授
2017/07-2022/08，中山大学，赌博app ，博士后，合作导师：李文均
2012/09–2017/06，中山大学，赌博app ，微生物学，理学博士，导师：刘玉焕
2008/09–2012/06，广东工业大学，环境科学与工程学院，生物工程，工学学士

承担课题

横向课题，酶制剂研发，在研，主持。
国家自然科学基金，青年项目，32000031，水通道介导水分子调控β-葡萄糖苷酶Ks5A7活性的机制研究，2021.01-2023.12，24万元，在研，主持。
广东省自然科学基金，面上项目，2020A1515010321，β-半乳糖苷酶Bgal1-3转糖基化产物聚合度调控的结构基础，2019.10-2022.09，10万元，在研，主持。
高校基本科研业务费，青年教师培育项目，19lgpy184，基于活性中心重塑策略解析β-葡萄糖苷酶Bglhi葡萄糖耐受性和催化活性同步优化的协同机制，2019.01-2020.12，15.33万，已结题，主持。
中国博士后科学基金，面上项目，2017M622859，β-葡萄糖苷酶Bgl6葡萄糖耐受性的调控位点及调控机制，2017.11-2019.06，5万元，已结题，主持。

论文专著

论文（#共同第一作者，*共同通讯作者）

14. Shuifeng Li^#, Lichuang Cao^#, Xiangpeng Yang, Xiangrui Wu, Shujing Xu and Yuhuan Liu. Simultaneously optimizing multiple properties of β-glucosidase Bgl6 using combined (semi-)rational design strategies and investigation of the underlying mechanisms. Bioresour. Technol. 2023, 374, 128792.

13. Lichuang Cao^#, Ran Chen^#, Xin Huang, Shuifeng Li, Sufang Zhang, Xiangpeng Yang, Zongmin Qin, Wei Kong, Wei Xie* and Yuhuan Liu*. Engineering of β-glucosidase Bgl15 with simultaneously enhanced glucose tolerance and thermostability to improve its performance in high-solid cellulose hydrolysis. J. Agric. Food Chem. 68, 5391-5401, 2020.

12. Zongmin Qin, Shuifeng Li, Xin Huang, Wei Kong, Xiangpeng Yang, Sufang Zhang, Lichuang Cao* and Yuhuan Liu*. Improving galactooligosaccharide synthesis efficiency of β-galactosidase Bgal1-3 by reshaping the active site with an intelligent hydrophobic amino acid scanning. J. Agric. Food Chem. 67, 11158-11166, 2019.

11. Xiwen Liu^#, Lichuang Cao^#, Jianhua Zeng, Yuhuan Liu*, Wei Xie*. Improving the cellobiose-hydrolysis activity and glucose-tolerance of a thermostable β-glucosidase through rational design. Int. J. Biol. Macromol. 136, 1052-1059, 2019.

10. Lichuang Cao, Shuifeng Li, Xin Huang, Zongmin Qin, Wei Kong, Wei Xie*, Yuhuan Liu*. Enhancing the thermostability of highly active and glucose-tolerant β-glucosidase Ks5A7 by directed evolution for good performance of three properties. J. Agric. Food Chem., 66(50), 13228-13235, 2018.

9. Xin Huang^#, Lichuang Cao^#, Zongmin Qin, Shuifeng Li, Wei Kong, Yuhuan Liu. Tat-independent secretion of polyethylene terephthalate hydrolase PETase in Bacillus subtilis 168 mediated by its native signal peptide. J. Agric. Food Chem., 66(50), 13217-13227, 2018.

8. Lichuang Cao^#, Guanghui Ren^#, Zongmin Qin, Xin Huang, Wei Kong, Zhijun Wang, Weiqu Liang, Xiaogang Bi*, Yuhuan Liu*. Improving the secretion yield of the β-galactosidase Bgal1-3 in Pichia pastoris for use as a potential catalyst in the production of prebiotic-enriched milk. J. Agric. Food Chem., 65(49), 10757-10766, 2017.

7. Panjiao Pang, Lichuang Cao, Yuhuan Liu, Wei Xie*, Zhong Wang*. Structures of a glucose-tolerant β-glucosidase provide insights into its mechanism. J. Struct. Biol., 198(3):154, 2017.

6. Xiwen Liu^#, Lichuang Cao^#, Xin-jiong Fan, Yuhuan Liu*, Wei Xie*. Engineering of a thermostable esterase Est816 to improve its quorum-quenching activity and the underlying structural basis. Sci. Rep., 6:38137, 2016.

5. Guang‑hui Ren^#, Lichuang Cao^#, Wei Kong, Zhi‑jun Wang, Yuhuan Liu. Efficient secretion of β-galactosidase Bgal1-3 via both Tat-dependent and Tat-independent pathways in Bacillus subtilis. J. Agric. Food Chem. 64(28): 5708-5716, 2016.

4. Lichuang Cao, Zhi‑jun Wang, Guang‑hui Ren, Wei Kong, Liang Li, Wei Xie and Yuhuan Liu. Engineering a novel glucose-tolerant β-glucosidase as supplementation to enhance the hydrolysis of sugarcane bagasse at high glucose concentration. Biotechnol. Biofuels. 8:202. 2015.

3. Lichuang Cao^#, Ran Chen^#, Wei Xie*, and Yuhuan Liu*. Enhancing the thermostability of feruloyl esterase EstF27 by directed evolution and the underlying structural basis. J. Agric. Food Chem. 63, 8225−8233. 2015.

2. Liang Li^#, Gang Li^#, Lichuang Cao, Guang-hui Ren, Wei Kong, Si-di Wang, Geng-shan Guo, and Yuhuan Liu*. Characterization of the cross-linked enzyme aggregates of a novel β-galactosidase, a potential catalyst for the synthesis of galacto-oligosaccharides. J. Agric. Food Chem. 63, 894-901. 2015.

1. Si-di Wang, Geng-shan Guo, Liang Li, Lichuang Cao, Ling Tong, Guang-hui Ren, Yuhuan Liu*. Identification and characterization of an unusual glycosyltransferase-like enzyme with β-galactosidase activity from a soil metagenomic library. Enzyme Microb. Technol, 57: 26-35, 2014.

专利

3. 刘玉焕，曹立创，张素芳，黄磊，杨向鹏，卫雅萱。热稳定性提高的β-半乳糖苷酶突变体及其应用，2023-06-19，中国,  202310731755.8。

2. 刘玉焕，曹立创，李水凤，黄欣，覃宗敏，孔伟。高热稳定性β-葡萄糖苷酶突变体及其应用，2018-11-21，中国, ZL201811393399.9。

1. 刘玉焕，曹立创，黄欣，李水凤，覃宗敏，孔伟。热稳定性提高的β-葡萄糖苷酶突变体及其应用，2018-11-21，中国, ZL201811393398.4。