性别:男
工作电话:020-39943513
职称:教授
电子邮件: [email protected]
导师类型: 博士生导师
学历: 博士
学科方向: 071001-植物学
0860-生物与医药
研究方向
（1）植物免疫信号转导（主要研究方向）
在电影《星际穿越》中，地球上的农作物先后被枯萎病团灭，只有玉米还在苦苦挣扎，人类终日只能吃土......现实中，病原菌引起的植物病害曾经对人类历史造成过深远影响。植物可以通过整合模式触发式免疫（PTI）和效应子触发式免疫（ETI），感知和抵御绝大多数病原菌。然而，少数开挂的病原菌能利用花样繁多的策略与植物大玩“躲猫猫”（逃避植物的免疫识别或阻断其免疫信号转导），以促进对植物的侵染。本课题组主要利用分子生物学、生物化学、细胞生物学、分子遗传学等手段探究植物感知病原菌以及免疫信号转导的分子机理。欢迎围观植物与病原菌这场不见硝烟的战争，学习安身立命的“三十六计”。
（2）植物靶向基因调控技术
基因缺失突变体的缺乏、基因功能冗余和基因缺失致死是植物基因功能研究中最主要的技术瓶颈。本课题组致力于建立和优化一套全方位的基因功能调控工具，可以实现高效的植物基因敲除、敲低与激活。
本课题组长期欢迎研究生与博士后青年人才加入！
个人经历
国家杰出青年科学基金获得者。1995年免试进入中山大学生物化学专业攻读本科，1999年免试进入中山大学生化与分子生物学专业攻读硕士，2002年起在中山大学生化与分子生物学专业攻读博士，于2005年获博士学位。2003-2004年在香港科技大学做访问学者（Visiting Scholar）。2005.11-2008.10在美国田纳西大学做博士后，2008.11-2014.8在美国哈佛医学院／麻省总医院任助理研究员（Research Fellow）。2014年9月起任赌博app-网络赌博软件 教授、博士生导师。2018年起任生物科学与技术系系主任。
2014、2015、2021年先后获得国家“海外高层次人才引进计划”青年项目、国家优秀青年科学基金、国家杰出青年科学基金资助。
讲授课程
目前主要讲授《生命科学史》、《大学生物》、《现代生命科学进展》、《新生科研研讨课》。
学术成就
以通讯作者或第一作者（含共同）在Nature、Nature Biotechnology、Nature Plants（4篇）、Cell Host & Microbe（2篇）、Molecular Plant（2篇）、The Plant Cell（3篇）、Trends in Plant Science等SCI期刊发表论文44篇。
担任aBIOTECH，Plant Methods， Frontiers in Plant Science期刊的Associate Editor，担任Nature Biotechnology、Nature Plants、Nature Communications、The Plant Cell、Molecular Plant 等期刊审稿人以及德国教育科学部研究基金（BMBF）海外评审专家。
承担课题
- 中山大学百人计划科研启动项目（2015.1-2016.12）
- 国家海外高层次人才计划青年项目（2016.1-2018.12）
- 国家自然科学基金委优秀青年科学基金 No.31522006（2016.1-2018.12）
- 国家自然科学基金委面上项目 No.31570276（2016.1-2019.12）
- 国家自然科学基金委面上项目 No.31770295（2018.1-2021.12）
- 国家重大科技专项子课题 No.2019ZX08010003-001-009（2019.1-2020.12）
- 广州市科技计划重点项目 No.201904020041（2019.4-2022.3）
- 国家自然科学基金委面上项目 No.31970278（2020.1-2023.12）
- 国家重点研发计划子课题 No.2019YFA0906202（2020.1-2024.12）
- 国家自然科学基金委杰出青年科学基金No.32125004（2022.1-2026.12）
- 广东省基础与应用基础研究重大项目No.2023B0303000022 (2023.4-2028.4)
论文专著
通讯作者及第一作者论文（按时间倒序）
2024
46. Wang FZ#,*, Bao Y#, Li Z, Xiong X, Li JF* (2024) A dual-function selection system enables positive selection of multigene CRISPR mutants and negative selection of Cas9-free progeny in Arabidopsis. aBIOTECH, 5: 140-150. (*通讯作者)
45. Ruan XM, Xiong X, Li JF* (2024) Identification and application of an exocarp-preferential promoter for genetic engineering of tomato fruit. Horticulture Research, 11: uhae035. (*通讯作者)
2023
44. Liu J#, Wang FZ#, Li C, Li Y, Li JF* (2023) Hidden prevalence of deletion-inversion bi-alleles in CRISPR-mediated deletions of tandemly arrayed genes in plants. Nature Communications，14： 6787. (*通讯作者)
43. Xiong X#, Liu K#*, Li Z, Xia FN, Ruan XM, He X, Li JF* (2023) Split complementation of base editors to minimize off-target edits. Nature Plants, 9: 1832-1847. (*最后通讯作者)
42. Wang FZ, Li JF* (2023) WeiTsing: a new face of Ca2+-permeable channels in plant immunity. Stress Biology, 3: 25. (*通讯作者)
2022
41. Li Y#, Xue J#, Wang FZ, Huang X, Gong BQ, Tao Y, Shen W, Tao K, Yao N, Xiao S, Zhou JM, Li JF* (2022) Plasma membrane-nucleo-cytoplasmic coordination of a receptor-like cytoplasmic kinase promotes EDS1-dependent plant immunity. Nature Plants, 8: 802-816. (*通讯作者)
40. Xiong X#, Li Z#*, Liang J, Liu K, Li C, Li JF* (2022) A cytosine base editor toolkit with varying activity windows and target scopes for versatile gene manipulation in plants. Nucleic Acids Research, 50: 3565-3580. (*最后通讯作者)
39. Shen W, Zhang X, Liu J, Tao K, Li C, Xiao S, Zhang W, Li JF* (2022) Plant elicitor peptide signalling confers rice resistance to piercing-sucking insect herbivores and pathogens. Plant Biotechnology Journal, 20: 991-1005. (*通讯作者)
2021
38. Shen W, Cao S, Liu J, Zhang W, Chen J*, Li JF* (2021) Overexpression of an Osa-miR162a derivative in rice confers cross-kingdom RNA interference-mediated brown planthopper resistance without perturbing host development. International Journal of Molecular Sciences, 22: 12652. (*最后通讯作者)
37. Yang Q, Guo J, Zeng H, Xu L, Xue J, Xiao S, Li JF* (2021) Receptor-like cytoplasmic kinase CDG1 negatively regulates Arabidopsis pattern-triggered immunity and is involved in AvrRpm1-induced RIN4 phosphorylation. The Plant Cell, 33: 1341-1360. (*通讯作者)
36. Guo J, Gong BQ, Li JF* (2021) Arabidopsis lysin motif/F-box-containing protein InLYP1 fine-tunes glycine metabolism by degrading glycine decarboxylase GLDP2. The Plant Journal, 106: 394-408. (*通讯作者)
35. Xiong X#, Liang J#, Li Z, Gong BQ, Li JF* (2021) Multiplex and optimization of dCas9-TV-mediated gene activation in plants. Journal of Integrative Plant Biology, 63: 634-645. (*通讯作者)
2020
34. Gong BQ#, Wang FZ#, Li JF* (2020) Hide-and-seek: Chitin-triggered plant immunity and fungal counterstrategies. Trends in Plant Science, 25: 805-816. (*通讯作者)
33. Wang FZ#, Zhang N#, Guo Y#, Gong BQ, Li JF* (2020) Split Nano luciferase complementation for probing protein-protein interactions in plant cells. Journal of Integrative Plant Biology, 62: 1065-1079. (*通讯作者)
32. Miao S, Liu J, Guo J, Li JF* (2020) Engineering plants to secrete affinity-tagged pathogen elicitors for deciphering immune receptor complex or inducing enhanced immunity. Journal of Integrative Plant Biology, 62: 761-776. (*通讯作者)
31. Xue J#, Gong BQ#, Yao X#, Huang X, Li JF* (2020) BAK1-mediated phosphorylation of canonical G protein alpha during flagellin signaling in Arabidopsis. Journal of Integrative Plant Biology，62: 690-701 . (*通讯作者)
30. Li Z, Xiong X, Li JF* (2020) The working dead: repurposing inactive CRISPR-associated nucleases as programmable transcriptional regulators in plants. aBIOTECH，1: 32-40. (*通讯作者)
2019
29. Gong BQ, Guo J, Zhang N, Yao X, Wang HB, Li JF* (2019) Cross-microbial protection via priming a conserved immune co-receptor through juxtamembrane phosphorylation in plants. Cell Host & Microbe， 26: 810-822. (*通讯作者)
28. Shen W#, Liu J#, Li JF* (2019) Type-II metacaspases mediate the processing of plant elicitor peptides in Arabidopsis. Molecular Plant, 12: 1524-1533. (*通讯作者)
27. Zhang D#, Zhang N#, Shen W*, Li JF* (2019) Engineered artificial microRNA precursors facilitate cloning and gene silencing in Arabidopsis and rice. International Journal of Molecular Sciences, 20: 5620. (*最后通讯作者)
26. Li Z, Xiong X, Wang F, Liang J, Li JF* (2019) Gene disruption through base editing-induced messenger RNA missplicing in plants. New Phytologist, 222: 1139-1148. (*通讯作者)
25. Xu L, Yao X, Zhang N, Gong BQ, Li JF* (2019) Dynamic G protein alpha signaling in Arabidopsis innate immunity. Biochemical and Biophysical Research Communications, 516: 1039-1045. (*通讯作者)
24. Huang X#, Xue J#, Wang F*, Li JF* (2019) Enhanced protoplast assay by transfecting PCR-assembled gene expression cassettes with telomeric repeats and thiophosphate modifications. Analytical Biochemistry, 569: 39-45. (*最后通讯作者)
2018
23. Li Z#, Xiong X#, Li JF* (2018) New cytosine base editor for plant genome editing. Science China-Life Sciences, 61: 1602-1603.(*通讯作者)
22. Zhang N#, Zhang D#, Chen SL, Gong BQ, Guo Y, Xu L, Zhang XN, Li JF* (2018) Engineering artificial microRNAs for multiplex gene silencing and simplified transgenic screen. Plant Physiology，178: 989-1001. (*通讯作者)
21. Liu J#, Liu B#, Chen S, Gong BQ, Chen L, Zhou Q, Xiong F, Wang M, Feng D, Li JF*, Wang HB*, Wang J* (2018) A tyrosine phosphorylation cycle regulates fungal activation of a plant receptor Ser/Thr kinase. Cell Host & Microbe，23: 241-253. (*共同通讯作者)
2017
20. Li Z#, Zhang D#, Xiong X, Yan B, Xie W, Sheen J, Li JF* (2017) A potent Cas9-derived gene activator for plant and mammalian cells. Nature Plants, 3: 930-936. (*通讯作者)
19. Gong BQ, Xue J, Zhang N, Xu L, Yao X, Yang QJ, Yu Y, Wang HB, Zhang D, Li JF* (2017) Rice chitin receptor OsCEBiP is not a transmembrane protein but targets to plasma membrane via a GPI anchor. Molecular Plant, 10: 767-770. (*通讯作者)
2016
18. Zhang D, Li Z, Yan B and Li JF* (2016) A novel RNA-guided RNA-targeting CRISPR tool. Science China-Life Sciences, 59:854-856.(*通讯作者)
17. Zhang D and Li JF* (2016) DNA-guided genome editing tool. Science China-Life Sciences, 59: 740-741. (*通讯作者)
16. Zhang D, Li Z and Li JF* (2016) Targeted gene manipulation in plants using the CRISPR/Cas technology. Journal of Genetics and Genomics, 43: 251-262. (*通讯作者)
2015
15. Zhang D, Li Z and Li JF* (2015) Genome editing: New antiviral weapon for plants. Nature Plants, 1: 15146. (*通讯作者)
博士后期间
14. Cheng ZY#, Li JF#, Niu Y, Zhang XC, Woody OZ, Xiong Y, Djonovic S, Millet Y, Bush J, McConkey BJ, Sheen J, Ausubel FM (2015) Pathogen-secreted proteases activate a novel plant immune pathway. Nature, 521: 213-216. (#共同第一作者)
13. Li JF*, Zhang D, Sheen J (2014) Epitope-tagged protein-based artificial microRNA screens for optimized gene silencing in plants. Nature Protocols, 9: 939-949. (*通讯作者)
12. Li JF, Norville JE, Aach J, McCormack M, Zhang DD, Bush J, Church GM, Sheen J (2013) Multiplex and homologous recombination-mediated genome editing in Arabidopsis and Nicotiana benthamiana using guide RNA and Cas9. Nature Biotechnology, 31: 688-691.
11. Li JF*, Chung HS, Niu Y, Bush J and Sheen J (2013) Comprehensive artificial microRNA screens for effective gene silencing in plants. The Plant Cell, 25: 1507-1522. (*通讯作者)
10. Liu B#, Li JF#, Ao Y#, Qu J, Li Z, Su J, Zhang Y, Liu J, Feng D, Qi K, He Y, Wang J and Wang HB (2012) Lysin motif-containing proteins LYP4 and LYP6 play dual roles in peptidoglycan and chitin perception in rice innate immunity. The Plant Cell, 24: 3406-3419. (#共同第一作者)
9. Li JF*, Bush J, Xiong Y, Li L and McCormack M (2011) High-throughput protein-protein interaction analysis in Arabidopsis mesophyll protoplasts by split firefly luciferase complementation. PLoS ONE, 6: e27364. (*通讯作者)
8. Li JF*, Li L and Sheen J (2010) A rapid and economical procedure for purification of plasmid or plant DNA with diverse applications in plant biology. Plant Methods, 6:1. (*通讯作者)
7. Li JF*, Park E, von Arnim AG and Nebenführ A* (2009) The FAST technique: a simplified Agrobacterium-based transformation method for transient gene expression analysis in seedlings of Arabidopsis and other plant species. Plant Methods, 5:6. (*共同通讯作者)
6. Li JF* and Nebenführ A (2008) Interdependence of dimerization and organelle binding in myosin XI. The Plant Journal, 55:478-490. (*通讯作者)
5. Li JF and Nebenführ A (2008) The tail that wags the dog: the globular tail domain defines the function of myosin V/XI. Traffic, 9:290-298.
4. Li JF and Nebenführ A (2007) Organelle targeting of myosin XI is mediated by two globular tail subdomains with separate cargo binding sites. Journal of Biological Chemistry, 282:20593-20602.
博士期间
3. Li JF, Qu LH and Li N (2005) Tyr152 plays a central role in catalysis of 1-aminocyclopropane-1-carboxylate synthase. Journal of Experimental Botany, 56:2203-2210.
2. Li JF, Qi R, Qu LH, Mattoo AK and Li N (2005) Cleavages of the carboxyl-terminus of LeACS2, a tomato ACC synthase isomer, by a 64-kDa tomato metalloprotease produce a truncated but active enzyme. Journal of Integrative Plant Biology, 47:1352-1363.
1. Li JF, Qu LH and Li N (2005) Isolation of a tomato protease that may be involved in proteolysis of 1-aminocyclopropane-1-carboxylate synthase. Journal of Integrative Plant Biology, 47:1220-1227.
第一作者或通讯作者论著
Li Z, Wang F, Li JF* (2019) Targeted transcriptional activation in plants using a potent dead Cas9-derived synthetic gene activator. Current Protocols in Molecular Biology, 127: e89. (*通讯作者)
Zhang N, Zhang D, Li JF* (2016) A simple protoplast-based method for screening potent artificial miRNA for maximal gene silencing in Arabidopsis. Current Protocols in Molecular Biology, 117: 26.9.1-26.9.9. (*通讯作者)
Li JF*, Zhang D and Sheen J (2015) Targeted plant genome editing via the CRISPR/Cas9 technology. Methods in Molecular Biology, 1284:239-255. (*通讯作者)
Li JF* and Zhang D (2014) Quantitative protein-protein interaction analysis by split firefly luciferase complementation in plant protoplasts. Current Protocols in Molecular Biology, 107: 20.9.1-20.9.8. (*通讯作者)
Li JF*, Zhang D and Sheen J (2014) Cas9-based genome editing in Arabidopsis and Tobacco. Methods in Enzymology, 546:459-472. (*通讯作者)
Li JF* and Sheen J (2012) DNA purification from multiple sources in plant research with homemade silica resins. Methods in Molecular Biology, 862:53-59. (*通讯作者)
Li JF and Nebenführ A (2010) FAST technique for Agrobacterium-mediated transient gene expression in seedlings of Arabidopsis and other plant species. Cold Spring Harbor Protocols, 5: pdb.prot5428.
