Prof. Dr. Shengfeng Huang
性别: 男
工作电话:020-39332958
职称:教授
个人主页: //duboapp.net/zh-hans/teacher/1213
ORCID page: //orcid.org/0000-0002-0968-1370
电子邮件: [email protected]
导师类型: 博士生导师
学科方向:
071004-水生生物学
071010-生物化学与分子生物学
0710Z1-生物信息学
Shengfeng Huang's Lab:
Research Fields: Comparative Immunology, Mucosal Biology, Bioinformatics (Multi-omics)
We are seeking or recruiting graduate students, postdoctoral fellows, or full-time research personnel who are interested or experienced in the following areas:
1. Mucosal physiology and immunogenetics of animals
2. Bioinformatics and immunogenomics of animals (immunomics/gut microbiome/single-cell transcriptomics/comparative genomics, etc.)
招生方向：生物与医药、生化与分子生物学、生物信息学、水生生物学
研究领域：比较免疫学、黏膜生物学、生物信息学（多组学）
因课题研究需要，招收或招聘有对以下工作有兴趣或有经验的研究生、博士后或者专职科研人员(长期有效)：
(1)动物的黏膜生理学和免疫遗传学，
(2)动物的生物信息学和免疫基因组学（免疫组/肠道微生物组/单细胞转录组/比较基因组学等）。
个人经历/Interests
Shengfeng Huang, is a professor in School of Life Sciences, Sun Yat-sen University, Guangzhou, China.
Our lab focuses on comparative immunogenetics and functional genomics, with a particular emphasis on studying mucosal functions in aquatic animals (mainly teleost fish) using both experimental and bioinformatic (multi-omics) approaches. Recently, our interests include the mechanisms of antibody diversity generation and vaccine responsiveness, the mucosal chitin barriers and microbial homeostasis, and the unique mucosal effector proteins and parasite immunity.
黄盛丰，赌博app-网络赌博软件 教授，水产动物疫病防控与健康养殖全国重点实验室PI，教育部青年长江学者，国家优秀青年基金获得者。主要研究方向为水生动物的免疫基因组学与遗传学，专注结合生物信息学手段发掘与研究水生动物（包括珍稀动物）的特色黏膜免疫蛋白、免疫蛋白的结构域重排机制以及抗体的体细胞重排机制，探索它们在水产动物的健康养殖、抗病抗逆、疫苗开发以及食品安全中的应用前景。以第一或通讯作者在Cell、PNAS(x2)、Nature Communications、Genome Research(x2)、EBMO Reports、Journal of Immunology(x2)、MPE(x2)、Free radical biology & medicine和JBC等期刊上发表研究论文30多篇。取得多项发明专利，开发软件与算法多项，曾获国家超算广州中心天河之星优秀应用奖。曾获国家自然科学奖二等奖、中国药学会科学技术一等奖、2019年广东省自然科学一等奖、2009年广东省科学技术一等奖，曾入选2016年中国科协生命科学领域十大进展。
讲授课程/Teaching
Immuology, Integrated Experiments for Biosciences, Experiments for Developmental Biology
免疫学，生物科学综合实验，发育生物学实验
学术成就
本研究团队长期从事动物的黏膜生理学和免疫遗传学研究，专注发掘和解析水生动物的特色黏膜防御策略及其机制，取得如下方面标志性成果。
一、我们揭示了水生动物从纯天然免疫步向适应性免疫的关键功能递进：发现适应性免疫的前身并非简单天然免疫，而是高度复杂与多样的天然免疫；揭示基因组高速演化是复杂天然免疫的关键驱动力；揭示脊椎动物基因组演化速度下降，是促使适应性免疫诞生的遗传背景；第一次证实有颌类脊椎动物的抗体V(D)J重排机制，来自无脊椎动物RAG转座子的转座机制；第一次发现硬骨鱼类具有非经典的抗体类别转换机制。相关成果发表在Cell、Nature Communications、Genome Research（x2）和Journal of Immunology等知名期刊上。曾入选中国生命科学领域十大进展，获中国药学会科学技术一等奖、广东自然科学一等奖和国家自然科学奖二等奖。
二、适应性免疫虽然强大，却不足以单独对抗病原，尤其是新病原的初次入侵或者老病原的大量入侵，因此，天然免疫是黏膜的第一道防线。我们发现和解析了多种水生动物特色天然免疫分子与机制。譬如发现了基于全新蛋白结构域ApeC的黏膜模式识别蛋白，揭示该类新型蛋白能够维持黏膜微生物稳态平衡，隔绝微生物在黏膜中定植，并调节黏膜免疫信号通路；又如第一次在反向遗传学层面上解析了鱼类肠道几丁质膜的基本生理功能，发现相比昆虫等无脊椎动物，鱼类几丁质膜不再是存活与繁殖的必需结构，但该膜形成的肠径向分区对消化吸收和菌群梯度分布非常重要，并且提供了额外的免疫屏障，是调节肠道消化和免疫的重要靶点。相关成果发表在PNAS、EMBO Reports、MPE、Frontiers in Immunology（x2）等知名期刊上。
三、水生动物各种特色黏膜天然免疫或适应性免疫机制需要适时激活和串联才能发挥最大效用。我们揭示了重要的免疫激活通路TLR/IL-1R通路，在不同水生动物和哺乳动物之间的关键功能差异；我们还第一次证实有免疫“主指挥者”之称的IL-17通路，来自TLR/IL-1R通路和cystine-knot生长因子通路的一次蛋白结构域重排事件，并且与大多数新生信号通路的独立演化不同，IL-17通路与它的两条供体通路一直保持交互作用，并因此主导了三条通路在不同动物门纲之间的功能模式差异化。相关成果发表在PNAS、Journal of Immunology、Free radical biology & medicine、Frontiers in Immunology和JBC等知名期刊上。
Selected Publications (*corresponding authors, #first authors)
代表性研究论文（*通讯作者，#第一作者）
Panwei Weng#, Mengjiao Lan#, Hao Zhang, Huiping Fan, Xiao Wang, Chenrui Ran, Zirui Yue, Jiaxuan Hu, Anlong Xu and Shengfeng Huang*. Both IRAK3 and IRAK1 Activate the MyD88-TRAF6 Pathway in Zebrafish. Journal of Immunology. 2024. //doi.org/10.4049/jimmunol.2400054
Shenghui Chen, Huiping Fan, Chenrui Ran, Yun Hong, Huixiong Feng, Zirui Yue, Hao Zhang, Pierre Pontarotti, Anlong Xu* and Shengfeng Huang*. The IL-17 Pathway Intertwines with Neurotrophin and TLR/IL-1R Pathways Since Its Domain Shuffling Origin. Proc Natl Acad Sci U S A. 2024 May 7; 121(19): e2400903121. //doi.org/10.1073/pnas.2400903121
Zirui Yue, Zhaoyu Fan, Hao Zhang, Buhan Feng, Chengyi Wu, Shenghui Chen, Jihua Ouyang, Huiping Fan, Panwei Weng, Huixiong Feng, Shangwu Chen, Meiling Dong, Anlong Xu* and Shengfeng Huang*. Differential roles of the fish chitinous membrane in gut barrier immunity and digestive compartments. EMBO Reports. 2023 Feb 28; 24(4): e56645. //doi.org/10.15252/embr.202256645
Jin Li, Shumin Liu, Yang Zhang, Qiuyun Huang, Hao Zhang, Jihua OuYang, Fan Mao, Huiping Fan, Wenjie Yi, Meiling Dong, Anlong Xu* and Shengfeng Huang*. Two Novel Mollusk Short-form ApeC-containing Proteins Act as Pattern Recognition Proteins for Peptidoglycan. Frontiers in Immunology. 2022 Oct 7; 13:971883. //doi.org/10.3389/fimmu.2022.971883
Jin Li, Yuhui Li, Zhaoyu Fan, Shenghui Chen, Xinyu Yan, Zirui Yue, Guangrui Huang, Shumin Liu, Hao Zhang, Meiling Dong, Anlong Xu* and Shengfeng Huang*. Two amphioxus ApeC-containing proteins bind to microbes and inhibit the TRAF6 Pathway. Frontiers in Immunology. 2021 Jul 30;12:715245. //doi.org/10.3389/fimmu.2021.715245
Yuhui Li, Jin Li, Xinyu Yan, Shenghui Chen, Chengyi Wu, Huiqing Huang, Yi Shi, Guangrui Huang, Meiling Dong, Anlong Xu* and Shengfeng Huang*. Broad distribution, high diversity and ancient origin of the ApeC-containing proteins. Molecular Phylogenetics and Evolution. 2021 Feb; 155:107009. //doi.org/10.1016/j.ympev.2020.107009
Yi Shi, Zhaoyu Fan, Guang Li, Lei Zhang, Zirui Yue, Xinyu Yan, Anlong Xu and Shengfeng Huang*. The family of amphioxus chitin synthases offers insight into the evolution of chitin formation in chordates. Molecular Phylogenetics and Evolution. 2020 Feb; 143: 106691. //doi.org/10.1016/j.ympev.2019.106691
Xinyu Yan, Shenghui Chen, Huiqing Huang, Ting Peng, Mengjiao Lan, Xia Yang, Meiling Dong, Shangwu Chen, Anlong Xu*, and Shengfeng Huang*. Functional variation of IL-1R associated kinases in the conserved MyD88-TRAF6 pathway during evolution. Journal of Immunology. 2020 Feb 15; 204(4):832-843. //doi.org/10.4049/jimmunol.1900222
Shengfeng Huang#, Xin Tao#, Shaochun Yuan#, Yuhang Zhang, Peiyi Li, Helen A. Beilinson, Ya Zhang, Wenjuan Yu, Pierre Pontarotti, Hector Escriva, Yann Le Petillon, Xiaolong Liu, Shangwu Chen, David G. Schatz, Anlong Xu. Discovery of an Active RAG Transposon Illuminates the Origins of V(D)J Recombination. Cell. 2016 Jun 30;166(1):102-14. //dx.doi.org/10.1016/j.cell.2016.05.032
Shengfeng Huang, Zelin Chen, Xinyu Yan, Ting Yu, Guangrui Huang, Pierre Antoine Pontarotti, Qingyu Yan, Hongchen Zhao, Jie Li, Ping Yang, Ruihua Wang, Rui Li, Xin Tao, Ting Deng, Yiquan Wang, Guang Li, Qiujin Zhang, Sisi Zhou, Leiming You, Shaochun Yuan, Yonggui Fu, Fenfang Wu, Meiling Dong, Shangwu Chen, Anlong Xu. Decelerated genome evolution in modern vertebrates revealed by analysis of multiple lancelet genomes. Nature communications. 2014 Dec 19; 5:5896. //dx.doi.org/10.1038/ncomms6896
Guangrui Huang#, Shengfeng Huang#, Xinyu Yan, Ping Yang, Jun Lia, Weiya Xua, Lingling Zhang, Ruihua Wang, Yingcai Yu, Shaochun Yuan, Shangwu Chen, Guangbin Luo and Anlong Xu. Two apextrin-like proteins mediate extracellular and intracellular bacterial recognition in amphioxus. Proc Natl Acad Sci U S A. 2014 Sep 16;111(37):13469-74. //dx.doi.org/10.1073/pnas.1405414111
Shengfeng Huang#, Zelin Chen#, Guangrui Huang, Ting Yu, Jie Li, Yonggui Fu, Shaochun Yuan, Shangwu Chen, Anlong Xu. HaploMerger: Reconstructing allelic relationships for polymorphic diploid genome assemblies. Genome Research. 2012 May; 22(8): 1581-1588. //dx.doi.org/10.1101/gr.133652.111
Shengfeng Huang#, Xin Wang#, Qingyu Yan, Lei Guo, Shaochun Yuan, Meiling Dong, Shangwu Chen, Anlong Xu. The evolution and regulation of the mucosal immune complexity in the basal chordate amphioxus. Journal of Immunology. 2011 Feb 15;186(4):2042-55. //dx.doi.org/10.4049/jimmunol.1001824
Shengfeng Huang#, Shaochun Yuan, Lei Guo, Yanhong Yu, Jun Li, Tao Wu, Tong Liu, Manyi Yang, Kui Wu, Huiling Liu, Jin Ge, Yingcai Yu, Huiqing Huang, Meiling dong, Cuiling Yu, Shangwu Chen, Anlong Xu. Genomic Analysis of the Immune Gene Repertoire of Amphioxus Reveals Extraordinary Innate Complexity and Diversity. Genome Research. 2008 Jul;18(7):1112-26. //dx.doi.org/10.1101/gr.069674.107
Other Publications (*corresponding authors, #first authors)
其它研究论文（*通讯作者，#第一作者）
Shenghui Chen, Xianan Fu, Ruihua Wang, Mingshi Li, Xinyu Yan, Zirui Yue, Shang-Wu Chen, Meiling Dong, Anlong Xu, Shengfeng Huang*. SUMO and PIAS repress NF-κB activation in a basal chordate. Fish and Shellfish Immunology. 2023 Jun;137: 108754. //doi.org/10.1016/j.fsi.2023.108754
Xianan Fu, Ruihua Wang, Mingshi Li, Xinyu Yan, Huiqing Huang, Jin Li, Shenghui Chen, Zirui Yue, Shangwu Chen, Yingqiu Li, Meiling Dong, Anlong Xu*, Shengfeng Huang*. Chordate PIAS proteins act as conserved repressors of the TRAF6 self-polyubiquitination. Developmental & Comparative Immunology. 2020 Mar; 104:103554. //doi.org/10.1016/j.dci.2019.103554
Muniba Faiza, Dongming Lan, Shengfeng Huang*, Yonghua Wang*. UPObase: An online database of unspecific peroxygenases. Database. 2019: baz122. //doi.org/10.1093/database/baz122
Muniba Faiza, Shengfeng Huang*, Dongming Lan and Yonghua Wang*. New insights on unspecific peroxygenases: superfamily reclassification and evolution. BMC Evolutionary Biology. 2019 Mar 13; 19(1):76. //doi.org/10.1186/s12862-019-1394-3
严信宇#, 黄盛丰#, 徐安龙, 人类抗体重排机制的起源, 科技导报, 2018;36(7):43-47. [北大中文核心期刊]
Shengfeng Huang*, Mingjin Kang and Anlong Xu. HaploMerger2: rebuilding both haploid sub-assemblies from high-heterozygosity diploid genome assembly. Bioinformatics. 2017 Aug 15;33(16):2577-2579. //doi.org/10.1093/bioinformatics/btx220.
Ruihua Wang#, Shengfeng Huang#, Xianan Fu, Guangrui Huang, Xinyu Yan, Zirui Yue, Shangwu Chen, Yingqiu Li and Anlong Xu. The conserved ancient role of chordate PIAS as a multilevel repressor of the NF-κB pathway. Scientific Reports. Scientific Reports. 2017 Dec 6;7(1):17063. //doi.org/10.1038/s41598-017-16624-7. #co-first author
Jose Ricardo Poole#, ShengFeng Huang#, Anlong Xu, Justine Bayet and Pontarotti Pierre. The RAG transposon is active through the deuterostome evolution and domesticated in jawed vertebrates. Immunogenetics. 2017 Jun;69(6):391-400. //doi.org/10.1007/s00251-017-0979-5.
Xinyu Yan, Wenbin Meng, Fenfang Wu, Anlong Xu and Shengfeng Huang*. The Nuclear DNA Content and Genetic Diversity of Lampetra morii. PLoS ONE. 2016 Jul 7;11(7):e0157494. //dx.doi.org/10.1371/journal.pone.0157494
Jie Li, Rui Li, Leiming You, Yonggui Fu, Anlong Xu* and Shengfeng Huang*. Evaluation of two statistical methods provides insights into the complex patterns of alternative polyadenylation site switching. PLoS ONE. 2015 Apr 14; 10(4):e0124324. //dx.doi.org/10.1371/journal.pone.0124324
Ping Yang#, Shengfeng Huang#, Xinyu Yan, Guangrui Huang, Xiangru Dong, Tingting Zheng, Dongjuan Yuan, Ruihuan Wang, Rui Li, Ying Tan and Anlong Xu. Origin of the phagocytic respiratory burst and its role in gut epithelial phagocytosis in a basal chordate. Free radical biology & medicine. 2014 May; 70C: 54-67. //dx.doi.org/10.1016/j.freeradbiomed.2014.02.007
Shengfeng Huang*, Jie Li, Anlong Xu, Guangrui Huang, Leiming You. Small insertions are more deleterious than small deletions in human genomes. Human Mutation. 2013 Sep 18; 34(12): 1642-1649. //dx.doi.org/10.1002/humu.22435
Zelin Chen#, Shengfeng Huang#, Yuxin Li, Anlong Xu. AliquotG: an improved heuristic algorithm for genome aliquoting. PLoS ONE. 2013 May 14; 8(5): e64279. //dx.doi.org/10.1371/journal.pone.0064279
Shengfeng Huang#, Ting Yu#, Zelin Chen, Shaochun Yuan, Shangwu Chen, Anlong Xu. More single-nucleotide mutations surround small insertions than small deletions in primates. Human Mutation. 2012 April 9; 33(7): 1099-1106. //dx.doi.org/10.1002/humu.22085
Huiqing Huang#, Shengfeng Huang#, Yanhong Yu, Shaochun Yuan, Rui Li, Xin Wang, Hongchen Zhao, Jun Li, Manyi Yang, Liqun Xu, Shangwu Chen, Anlong Xu. Functional characterization of a ficolin-mediated complement pathway in amphioxus. Journal of Biological Chemistry. 2011 Oct 21; 286(42):36739-48. //dx.doi.org/10.1074/jbc.M111.245944
Shengfeng Huang#, Haozheng Tian, Zelin Chen, Ting Yu, Anlong Xu. The evolution of vertebrate tetraspanins: gene loss, retention, and massive positive selection after whole genome duplications. BMC Evolutionary Biology. 2010;10:306. //dx.doi.org/10.1186/1471-2148-10-306
Shaochun Yuan#, Shengfeng Huang#, Wei Zhang, Tao Wu, Meiling Dong, Yanhong Yu, Tong Liu, Kui Wu, Huiling Liu, Manyi Yang, Hongwei Zhang, Anlong Xu. An amphioxus TLR with dynamic embryonic expression pattern responses to pathogens and activates NF-kappaB pathway via MyD88. Molecular Immunology. 2009 Jul;46(11-12):2348-56. //dx.doi.org/10.1016/j.molimm.2009.03.022
Shengfeng Huang#, Shaochun Yuan, Meiling Dong, Jing Su, Cuiling Yu, Xiaojin Xie, Yang Shen, xuesong Yu, Yanhong Yu, Shangwu Chen , Shicui Zhang, Pierre Pontarotti, Anlong Xu. The phylogenetic analysis of tetraspanins projects the evolution of cell-cell interactions from unicellular to multicellular organisms. Genomics. 2005 Dec; 86(6):674-684. //doi.org/10.1016/j.ygeno.2005.08.004
